Genomic DNA extraction from sapwood of Pinus roxburghii for polymerase chain reaction studies
نویسندگان
چکیده
منابع مشابه
A Simple and Rapid Leaf Genomic DNA Extraction Method for Polymerase Chain Reaction Analysis
In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...
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متن کاملPurification of genomic DNA extracted from environmental sources for use in a polymerase chain reaction.
The ability to amplify genomic DNA in a polymerase chain reaction (PCR) is dependent upon the purity of the DNA template. Environmental genomic DNA often contains contaminants (e.g., polyphenols, humic acids, polysaccharides) that reduce template purity and can be difficult to remove, thereby inhibiting PCR amplification. There is thus a need for a method to purify extracted genomic DNA without...
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BACKGROUND Pinus roxburghii Sarg. Is one of 3 species of pine found in Nepal, the oil of which is traditionally used to treat cuts, wounds, boils, and blisters. OBJECTIVE To obtain, analyze, and examine the anti-microbial and cytotoxic activities of the essential oils of P. roxburghii. MATERIALS AND METHODS Three plant parts (cone, needle, and bark) of Pinus roxburghii were collected in Bir...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2013
ISSN: 1684-5315
DOI: 10.5897/ajb12.2733